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Collection

The collection kit for the umbilical cord blood sample will arrive directly at your home immediately after you have filled the enrollement form. The collection kit contains the following:

  • 1 insulated box for sending the cord blood back to Salveo
  • 1 sterile bag for the collection of the blood sample (250 ml capacity) complete with a blood collection needle with safety guard contained in a sealed package (which must be opened only in the delivery room)
  • 5 blood collection tubes BD Vacutainer for the collection of maternal blood
  • 1 airtight container for the blood bag
  • 1 piece of absorbent tissue (essential for transportation)
  • 1 (distance) piece made of cardboard
  • refrigerated packs (gel packs) for transportation
  • 1 temperature datalogger

Forms and instructions:

  • Covering letter
  • Clinical history form and family medical history form
  • Description of the procedure for the collection of the umbilical cord blood
  • Collection protocol Identification data
  • Description of the procedure for correct storage of the collection kit
  • Umbilical cord blood shipping instructions
  • DHL air-waybill for shipment of the umbilical cord blood

Proforma invoice for the delivery after birth (if the sample is sent from outside Switzerland)

Everything will be closed in an insulated casing patented for the transport, according to applicable international security

standards.

embryonic cord blood lab processing

Processing

Processing involves a series of steps to remove excess red blood cells (RBCs) and plasma and bring each collection down to a uniform single unit volume of 20 ml. (large collections may be frozen in two separate 20 ml. containers). A uniform volume assures that freezing conditions are uniform for all units and greatly facilitates storage and automation.

Upon arrival in the Processing Laboratory, cord blood units are “logged-in” to the Salveo database and weighed to estimate the collected blood volume. The cord blood is transferred using a sterile-docking device, from the collection bag into a sterile, disposable processing bag set in preparation for its automated processing.

Before and after processing, small samples are removed to count the total nucleated cells (TNC) and the number of nucleated cells that are positive for CD34, a marker of a very early class of cells that include the hematopoietic stem cells. These samples are removed within plastic bubbles (pre-set in the tubing), sealed off from the processing bag set without exposing the products to possible infectious agents in the air of the laboratory. The bag set containing the cord blood unit is placed into the computerized device, which is automatically centrifuged twice. The first centrifugation stratifies the cellular components [RBCs, white blood cells (WBC) and platelets] separated from the plasma. The second spin harvests the blood components into the three different bags of the XXX sterile bag set: RBC bag, freezing bag [containing the WBCs, predominantly the mononuclear cells (MNCs)] and plasma bag.

The freezing bag contains the 20 ml final product (on average, more than 90% of the original MNCs and stem cells in some of the plasma, along with some red cells). Cold, sterile, Dimethylsulfoxide (DMSO, 5 ml of a 50% solution) is then added slowly. DMSO is a kind of “anti-freeze” or cryoprotectant that prevents ice crystals from forming inside of and destroying cells during freezing. It is also protective when the unit is eventually thawed.